ht 8 oh dg elisa ii kit Search Results


ht 8 oxo dg elisa kit ii assay  (R&D Systems)
93
R&D Systems ht 8 oxo dg elisa kit ii assay
Involvement of the PAR-2/NF-κB axis in increased tissue factor expression in AAA. ( A ) Fluorescent detection of cellular oxidant levels using CM-H 2 DCFDA ( n = 9). ( B ) Levels of 8-oxo-7,8-dihydro-2′-deoxyguanosine <t>(8-oxo-dG)</t> in cell supernatants ( n = 9–10). ( C ) Seahorse respiratory measurements, namely basal respiration, ATP-linked respiration, proton-linked respiration, maximum respiratory capacity, and reserve capacity in AAA-SMCs compared with HA-SMCs ( n = 9–12). ( D ) TNF-α mRNA levels in SMCs cultured from HA and AAA aortas ( n = 8). ( E ) Representative images of Western blot to assess levels of PAR-2, phosphorylated and total IKK, and NF-κB in SMCs. ( F–H ) Quantification of PAR-2, p-IKK/IKK, and p-NF-κB/NF-κB band intensities ( n = 5–6). ( I ) Immunofluorescence staining of α-SMA (red) and p-NF-κB (green) in aortic tissues. Nuclei were counterstained using DAPI. ( J ) Immunofluorescence staining of p-NF-κB in SMCs. ( K, L ) TF activity at the surface of SMCs incubated for 4 hours with the PAR-2 antagonist GB83 or the inhibitor of NF-κB activation BAY 11–7082 (BAY). Values are normalized by TF activity in the absence of GB83 or BAY ( n = 5). Data represent means ± SD. Groups were statistically analyzed by the Mann–Whitney test.
Ht 8 Oxo Dg Elisa Kit Ii Assay, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht+8+oh+dg+elisa+ii+kit/HT+8-oxo-dG+ELISA+Kit+II/pmc12932002-73-11-17
Average 93 stars, based on 1 article reviews
ht 8 oxo dg elisa kit ii assay - by Bioz Stars, 2026-10
93/100 stars
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ohdg elisa kit quantikine  (R&D Systems)
94
R&D Systems ohdg elisa kit quantikine
Involvement of the PAR-2/NF-κB axis in increased tissue factor expression in AAA. ( A ) Fluorescent detection of cellular oxidant levels using CM-H 2 DCFDA ( n = 9). ( B ) Levels of 8-oxo-7,8-dihydro-2′-deoxyguanosine <t>(8-oxo-dG)</t> in cell supernatants ( n = 9–10). ( C ) Seahorse respiratory measurements, namely basal respiration, ATP-linked respiration, proton-linked respiration, maximum respiratory capacity, and reserve capacity in AAA-SMCs compared with HA-SMCs ( n = 9–12). ( D ) TNF-α mRNA levels in SMCs cultured from HA and AAA aortas ( n = 8). ( E ) Representative images of Western blot to assess levels of PAR-2, phosphorylated and total IKK, and NF-κB in SMCs. ( F–H ) Quantification of PAR-2, p-IKK/IKK, and p-NF-κB/NF-κB band intensities ( n = 5–6). ( I ) Immunofluorescence staining of α-SMA (red) and p-NF-κB (green) in aortic tissues. Nuclei were counterstained using DAPI. ( J ) Immunofluorescence staining of p-NF-κB in SMCs. ( K, L ) TF activity at the surface of SMCs incubated for 4 hours with the PAR-2 antagonist GB83 or the inhibitor of NF-κB activation BAY 11–7082 (BAY). Values are normalized by TF activity in the absence of GB83 or BAY ( n = 5). Data represent means ± SD. Groups were statistically analyzed by the Mann–Whitney test.
Ohdg Elisa Kit Quantikine, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht+8+oh+dg+elisa+ii+kit/HT+8-oxo-dG+ELISA+Kit+II/pmc10995875-127-2-6
Average 94 stars, based on 1 article reviews
ohdg elisa kit quantikine - by Bioz Stars, 2026-10
94/100 stars
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8 hydroxy 2 deoxyguanosine  (R&D Systems)
94
R&D Systems 8 hydroxy 2 deoxyguanosine
Involvement of the PAR-2/NF-κB axis in increased tissue factor expression in AAA. ( A ) Fluorescent detection of cellular oxidant levels using CM-H 2 DCFDA ( n = 9). ( B ) Levels of 8-oxo-7,8-dihydro-2′-deoxyguanosine <t>(8-oxo-dG)</t> in cell supernatants ( n = 9–10). ( C ) Seahorse respiratory measurements, namely basal respiration, ATP-linked respiration, proton-linked respiration, maximum respiratory capacity, and reserve capacity in AAA-SMCs compared with HA-SMCs ( n = 9–12). ( D ) TNF-α mRNA levels in SMCs cultured from HA and AAA aortas ( n = 8). ( E ) Representative images of Western blot to assess levels of PAR-2, phosphorylated and total IKK, and NF-κB in SMCs. ( F–H ) Quantification of PAR-2, p-IKK/IKK, and p-NF-κB/NF-κB band intensities ( n = 5–6). ( I ) Immunofluorescence staining of α-SMA (red) and p-NF-κB (green) in aortic tissues. Nuclei were counterstained using DAPI. ( J ) Immunofluorescence staining of p-NF-κB in SMCs. ( K, L ) TF activity at the surface of SMCs incubated for 4 hours with the PAR-2 antagonist GB83 or the inhibitor of NF-κB activation BAY 11–7082 (BAY). Values are normalized by TF activity in the absence of GB83 or BAY ( n = 5). Data represent means ± SD. Groups were statistically analyzed by the Mann–Whitney test.
8 Hydroxy 2 Deoxyguanosine, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ht+8+oh+dg+elisa+ii+kit/HT+8-oxo-dG+ELISA+Kit+II/pm37888727-50-2-10
Average 94 stars, based on 1 article reviews
8 hydroxy 2 deoxyguanosine - by Bioz Stars, 2026-10
94/100 stars
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Image Search Results


Involvement of the PAR-2/NF-κB axis in increased tissue factor expression in AAA. ( A ) Fluorescent detection of cellular oxidant levels using CM-H 2 DCFDA ( n = 9). ( B ) Levels of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dG) in cell supernatants ( n = 9–10). ( C ) Seahorse respiratory measurements, namely basal respiration, ATP-linked respiration, proton-linked respiration, maximum respiratory capacity, and reserve capacity in AAA-SMCs compared with HA-SMCs ( n = 9–12). ( D ) TNF-α mRNA levels in SMCs cultured from HA and AAA aortas ( n = 8). ( E ) Representative images of Western blot to assess levels of PAR-2, phosphorylated and total IKK, and NF-κB in SMCs. ( F–H ) Quantification of PAR-2, p-IKK/IKK, and p-NF-κB/NF-κB band intensities ( n = 5–6). ( I ) Immunofluorescence staining of α-SMA (red) and p-NF-κB (green) in aortic tissues. Nuclei were counterstained using DAPI. ( J ) Immunofluorescence staining of p-NF-κB in SMCs. ( K, L ) TF activity at the surface of SMCs incubated for 4 hours with the PAR-2 antagonist GB83 or the inhibitor of NF-κB activation BAY 11–7082 (BAY). Values are normalized by TF activity in the absence of GB83 or BAY ( n = 5). Data represent means ± SD. Groups were statistically analyzed by the Mann–Whitney test.

Journal: Thrombosis and Haemostasis

Article Title: NF-κB-Mediated Upregulation of Tissue Factor Contributes to the Procoagulant Phenotype of Smooth Muscle Cells from Abdominal Aorta Aneurysm in Human

doi: 10.1055/a-2665-2510

Figure Lengend Snippet: Involvement of the PAR-2/NF-κB axis in increased tissue factor expression in AAA. ( A ) Fluorescent detection of cellular oxidant levels using CM-H 2 DCFDA ( n = 9). ( B ) Levels of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dG) in cell supernatants ( n = 9–10). ( C ) Seahorse respiratory measurements, namely basal respiration, ATP-linked respiration, proton-linked respiration, maximum respiratory capacity, and reserve capacity in AAA-SMCs compared with HA-SMCs ( n = 9–12). ( D ) TNF-α mRNA levels in SMCs cultured from HA and AAA aortas ( n = 8). ( E ) Representative images of Western blot to assess levels of PAR-2, phosphorylated and total IKK, and NF-κB in SMCs. ( F–H ) Quantification of PAR-2, p-IKK/IKK, and p-NF-κB/NF-κB band intensities ( n = 5–6). ( I ) Immunofluorescence staining of α-SMA (red) and p-NF-κB (green) in aortic tissues. Nuclei were counterstained using DAPI. ( J ) Immunofluorescence staining of p-NF-κB in SMCs. ( K, L ) TF activity at the surface of SMCs incubated for 4 hours with the PAR-2 antagonist GB83 or the inhibitor of NF-κB activation BAY 11–7082 (BAY). Values are normalized by TF activity in the absence of GB83 or BAY ( n = 5). Data represent means ± SD. Groups were statistically analyzed by the Mann–Whitney test.

Article Snippet: Extracellular 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxo-dG) was measured in cell culture supernatants using the HT 8-oxo-dG ELISA Kit II assay (R&D Systems).

Techniques: Expressing, Cell Culture, Western Blot, Immunofluorescence, Staining, Activity Assay, Incubation, Activation Assay, MANN-WHITNEY